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School of Pharmacy research laboratories and core facilities
School of Pharmacy

»Research Facilities NMR Core Facility

The research laboratories and core facilities (18,200 sq. ft.) are located on the 2nd floor of 9501 Jeronimo Road in Irvine. The facilities include space for dedicated tissue cultures, NMR, microscopy, structural biology, autoclave, and procedure and environmental rooms.

Formal courses for students on laboratory instrumentation and techniques are taught by the Core Facility Manager. Both basic and advanced analytical instrumentation required for biomedical, pharmaceutical, and biotechnological research are available for shared usage. Facility services are available to private companies on a fee-for-service basis.

Equipment


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Chromatography

Shimadzu Prep HPLC  

Preparative HPLC Systems by Shimadzu.

Learn more about the Shimadzu Prep HPLC.

 

NGC Medium-Pressure Chromatography Systems

Bio-Rad NGC Medium-Pressure Chromatography Systems (FPLC) for biomolecules purification.

Learn more about the Bio-Rad NGC Medium-Pressure Chromatography Systems.

 ngc medium pressure chromatography system


Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC)

The UHPLC is an analytical instrument used to identify and characterize the compounds in a mixture under a very high pressure (19,000 psi.) and to provide fast and higher resolution. Different detectors were included in the upgrade.

Learn more about the Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC).

 Shimadzu Nexera X2 Chromatography

Spatial Biology

GeoMx Digital Spatial Profiler

GeoMx is the only spatial biology platform that non-destructively profiles expression of RNA and protein from distinct tissue compartments and cell populations with an automated and scalable workflow that integrates with standard histology staining.

Learn more about the GeoMx Digital Spatial Profiler.


nCounter Pro Analysis Sytem

The nCounter Pro Analysis System delivers highly reproducible expression data on 800+ targets with zero enzymatic steps using a simple automated workflow that requires only 15 minutes of hands-on time. Compatible with even the most difficult sample types, such as FFPE, you get robust performance that drives biomarker discovery and development.

Learn more about the nCounter Pro Analysis System.

Cytometers

FACSymphony A1

This benchtop flow cytometer packs premium high-end BD FACSymphony instrument technology to meet a broad spectrum of research needs, from small particle research to 16-color immunophenotyping.

Learn more about the BD FACSymphony A1 Cell Analyzer.


BD FACSAria Fusion Flow Cytometer

The cell sorter that delivers exceptional multicolor performance

Learn more about the FACSAria Fusion Flow Cytometer.



BD LSRFortessa X-20 Cell Analyzer

 A core lab flow cytometer.

Learn more about the BD LSRFortessa X-20 Cell Analyzer.

Microscopes

Nikon A1R Confocal Microscope

Nikon A1R confocal microscope system provides high-resolution imaging of up to 4096 x 4096 pixels with a galvano (non-resonant) scanner and increased light detection efficiency realizes high image quality.

 Nikon Ti-E Inverted Microscope

Nikon Ti-E Inverted Microscope

The Nikon Ti-E is a fully motorized for automated multi-mode image techniques and acquisition. The Ti offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. 

Learn more about the Nikon Ti-E Inverted Microscope.

 Nikon A1R Confocal Microscope

PCR Detection Systems

C11000 Touch and CFX96 Touch™ Real-Time PCR Detection System

Bio-Rad CFX96™ Optical Reaction Module converts the C1000 Touch™ Thermal Cycler chassis into the powerful and precise CFX96 Touch Real-Time PCR Detection System.

Learn more about the C11000 Touch and CFX96 Touch Real-Time PCR Detection System.		 C11000 touch andnCFX96 detection system

QuantStudio 3 and 5 Real-Time PCR Systems

Designed for real-time data analysis and data sharing.

Learn more about the QuantStudio 3 and 5 Real-Time PCR Systems. 

  
 

Spectrofluorophotometers

RF-5301PC Spectrofluorophotometer
            
Compared to absorbance methods, fluorescence sensitivity is tens to thousands times better - this means that you can analyze nanogram to picogram samples with great results. Fluorescence can be used also to identify a specific molecule in a complex background. When the compound of interest does not exhibit natural fluorescence, functional group-specific probes may be used to label the compound.

Learn more about RF-5301PC Spectrofluorophotometer.		 RF-530 Spectroflourometer

Spectrometers

ALPHA FT-IR Spectrometer/Platinum ATR

Bruker Alpha FTIR interferometer with platinum ATR is based on Bruker Optics` patented RockSolid™ design. It offers full FT-IR sampling flexibility. ALPHA's Platinum ATR single reflection diamond ATR module is easy to use for routine analysis. A variety of sampling options for temperature controlled measurements and liquid flow through analysis complete the versatility of the Platinum ATR.

 Alpha FT-IR Spectrometier

Bruker Avance III HDTM 400, High Performance Digital Nuclear Magnetic Resonance (NMR) Spectrometer

NMR spectroscopy is used to determine the chemical structures, interaction of molecules, and composition of mixtures. Upgrade includes SampleCASE™ automated sample changer and Prodigy™ ultra-high sensitivity multinuclear Broadband CryoProbe (5 mm sample diameter), with high sensitivity 2H lock channel.

 

 Bruker Avance III HDTM 400

Shimadzu BioSpec-nano

Shimadzu BioSpec-nano is a micro-volume UV/VIS spectrophotometer. It offers superior detection limits, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and photometric measurements. Quantitation can be rapidly and simply carried out with very small samples ranging from 1µL-2µL And, with dedicated functions, such as automated sample mounting and wiping.

Learn more about Shimadzu BioSpec-nano.		 Shimadzu BioSpec Nano machine

UV-2600 UV-Vis Spectrophotometer

Shimadzu UV-2600 is a compact universal, research-grade UV/VIS spectrophotometer that can be used in a wide range of fields, and easily expanded to suit the measurement objective. By using the optional integrating sphere, the measurement wavelength range of the UV-2600 can be extended to the near-infrared region of 1400 nm.

Learn more about the UV-2600 UV-Vis Spectrophotometer.		 UV Spectrophotometer machine

Mass Spectrometers

EVOQ Triple Quadrupole LC-TQ Mass Spectrometer

Bruker EVOQ triple quadrupole for liquid chromatography (LC-TQ) is exceptional sensitivity, precision, accuracy, linearity, and a wide dynamic range for your multiple reaction monitoring (MRM) assays. Key application this technology can be used includes food Testing, environmental Analysis, and pharmacokinetics.

Learn more about EVOQ Triple Quadrupole LC-TQ Mass Spectrometer.

 EVOQ Triple Quad Rupole LC-TQ Mass Spectrometer machine

Impact II™, ESI–QTOF Mass Spectrometer

Bruker Impact II™ is UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight) mass spectrometry product line with industry-leading > 50,000 Full-Sensitivity Resolution (FSR). It opens up enhanced analytical performance levels for all applications where trace analysis from complex, high-background matrices is a challenge — such as biomarker research, identification of impurities, or residue screening.

Learn more about Impact II, ESI-QTOF Mass Spectrometer.		 ImpactII ESI-QTOF Mass Spectrometer machine

Shimadzu LCMS 2020 Single Quadrupole Liquid Chromatograph Mass Spectrometer (LC/MS)

LCMS is used to separate, detect, characterize, and analyze small molecules, proteins, metabolites, pharmaceuticals, and natural products.

 Shimadzu LCMS 2020 Single Quadrupole Spectrometer

Autoflex Speed MALDI-TOF Mass Spectrometer

Bruker Autoflex Speed MALDI-TOF and TOF/TOF technology is easy-to-use; robust systems for both traditional MALDI applications (reliable and detailed protein/peptide characterization, and polymer analysis) as well as cutting edge work (high resolution MALDI Tissue Imaging, glycan analysis. Further improved smartbeam™-II laser technology with up to 2,000 Hz repetition rate is a must for MALDI Tissue Imaging and delivers superior performance for any Proteomics project.

 Autoflex Spectrometer Autoflex Spectrometer machine

Synthesizers

Tribute Peptide Synthesizer

Protein Technologies model Tribute peptide synthesizer is a compact benchtop design with chemical-resistant touchscreen and unique Intellisynth UV-Monitoring and Feedback Control system.

 Tribute Peptide Synthesizer

X-ray Crystallography

Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction

The METALJET for X-ray diffraction delivers the smallest and most intensive X-ray beam of any home lab X-ray source to meet the ever increasing demands of modern structural biology including protein crystallography and Small Angle X-ray Scattering.  

Learn more about Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction.		 MetalJet x-ray crystallographer

Electrophysiology

QPatch – Automated Patch Clamp

QPatch is a fully automated patch clamp system designed to perform parallel gigaseal ion channel recordings of 16 or 48 cells. 

Learn more about the QPatch Automated Patch Clamp.

Analytical Core Facility

The CUSP Analytical Core Facility, established in 2015, is located at Chapman University School of Pharmacy, the first School of Pharmacy in Orange County, California. The facility is equipped with both basic and advanced analytical instrumentations required in biomedical, pharmaceutical, and biotechnological research. The facility provides research and training support to researchers from Chapman University and other academic/non-profit institutions and private companies.


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Equipment Training

All first-time equipment users must obtain training from the Core Facility staff on properly operating the equipment. The mandatory instrument operator training is provided at no cost. After completing the training users of the facility have independent access to all technologies located in the facility. Full operator-assisted analysis service is available on an additional fee-for-service basis.

Consumable Supplies

The Core Facility provides training, access to the equipment, and any needed assistance. It does not provide consumable supplies, such as solvents, other chemicals, reagents, standards, culture flasks, media, filters, chromatographic columns, pipettes, tips, tissue paper, etc., which are the responsibility of the users. If used, it will be billed in addition to the instrument usage rate.

Request for Access

All first-time users are required to complete the online registration form approved by the principal investigator and sent by e-mail to the Core Facility Coordinator. Unregistered usage of the Facility is prohibited. The Facility is open during normal business hours (8:30 am to 5:30 pm). After-hour access can be granted when specifically requested and approved by the Facility Coordinator. Summer Undergraduate Research Fellows will generally not have after-hour access without supervision by a trained individual from the same laboratory.

Equipment Fees

  • All Chapman Investigators have free access
  • One-time submission or use: create a Purchase Order
  • Multiple submissions or use: create a blanket order
  • A minimum unit rate per hour or per sample will be applied

An invoice will be provided for all facility usage on a monthly basis

Please use this link for equipment fees.

Acknowledgement of Facility

All Core Facility users are required to acknowledge specific services in their scientific meeting abstracts and publications: The research was made possible by the use of the CUSP Research Core Facility, supported by the Office of Associate Dean of Research, Graduate Studies, and Global Affairs

NMR Core Facility

Welcome to the NMR Research Core Facility of the School of Pharmacy at the Chapman University. We offer various services to researchers from Chapman University and external users. We can provide training and advise researchers on how to use NMR spectrometers efficiently. We also can run experiments requested by users.

Fee: Researchers and students of Chapman University have reduced base rates as specified below.

For outside users, current base rates are $20/proton and $50/carbon experiment (tube and solvent included).

Our facility is equipped with a 400 MHz Bruker NMR spectrometer.

  • 5 mm broadband observe (BBO) probe:
    • 1H (outer coil); 15N-31P and 19F (inner coil).
  • Prodigy broadband observes CryoProbeTM:
    • 1H/19F (outer coil); 15N-31P (inner coil).
  • Z-axis pulsed field gradient capability.
  • Autosampler for automated analysis of up to 24 samples.


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General Rules and Requirements

  • Chapman researchers can use the spectrometer for educational or research projects without the charge.
  • Students, assistants, and postdocs, who would like to use the spectrometer, should provide a written request from their PI (Chapman University faculty).
  • All users should demonstrate sufficient proficiency in operating the spectrometer. User training is available upon request.
  • All users can analyze their samples with the room-temperature BBO (broadband observation) or Prodigy CryoProbeTM. Please contact Dr. Maslennikov in advance if you need to use the CryoProbe.
  • Please do not bring any radioactive samples – we will not allow you to analyze them under any circumstances.
  • All self-service users should provide their own tubes, caps, and NMR-compatible solvents. We recommend Norell 508-UP-7 / Wilmad SP528 or better tubes for quantitative analysis and Norell SVCP-5-178 or better tubes for HTP experiments.
  • Other rules for sample preparation:
    • Use PTFE caps for volatile solvents.
    • Do not use broken tubes and/or damaged caps.
    • Solvent/sample length in the NMR tube: minimum 4 cm, maximum 5 cm.
    • If your tube broke accidentally in the magnet, let us know immediately, and do not try to recover the tube – the probe can be damaged.
  • If you want to use special tubes, please let us know in advance.
  • We can provide you with appropriate HTP NMR tubes and caps if you return them clean and undamaged.
  • The facility can provide necessary equipment for sample preparation (pipette samplers, tips, hand centrifuge for NMR tubes) and temporary storage (+4C, -20C).
  • If you need any additional equipment to handle your samples, please bring it with you or let us know in advance.

Current Rates for NMR Spectroscopy Services

Internal users self-service: Free of charge.

Internal and External users non-self-service (rates per sample): Please contact Dr. Innokentiy "Inno" Maslennikov at maslenni@chapman.edu 

Facility Acknowledgement

In the event you use data collected in our Facility for publication, please include  acknowledgment of Chapman University School of Pharmacy NMR Core Facility assistance.

Thank you.