School of Pharmacy  research laboratories and core facilities
School of Pharmacy

» Research Facilites

CORE FACILITIES

The research laboratories and core facilities (10,200 sq.ft) are located on the 2nd floor of 9501 Jeronimo Road in Irvine. The facilities include space for dedicated tissue culture, NMR, autoclave, and procedure and environmental rooms.

A 4,000 sq. ft. vivarium is on the first floor of the 9401 building. A second Core Lab Facility was completed in August 2015. Formal courses for students on laboratory instrumentation and techniques are taught by the Core Facility Manager. Both basic and advanced analytical instrumentation required for biomedical, pharmaceutical, and biotechnological research are available for shared usage. Facility services are available to private companies on a fee-for-service basis.

EQUIPMENT


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+ - Chromatography

ngc medium pressure chromatography system

NGC Medium-Pressure Chromatography Systems

Bio-Rad NGC Medium-Pressure Chromatography Systems (FPLC) for biomolecules purification.

Learn more about the Bio-Rad NGC Medium-Pressure Chromatography Systems.

Shimadzu Nexera X2 Chromatography

Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC)

The UHPLC is an analytical instrument used to identify and characterize the compounds in a mixture under a very high pressure (19,000 psi.) and to provide fast and higher resolution. Different detectors were included in the upgrade.

Learn more about the Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC).

+ - Cytometers

BD FACSVerse flow cytometer

BD FACSVerse FLOW CYTOMETER

Flow cytometry is used for cell counting, cell sorting, and detection of biomarkers with the use of laser in the fluidic media containing a cell suspension. 

Learn more about the BD FACSVerse Flow Cytometer.

+ - Generators

geneerator

Peak Scientific Nitrogen Generator - Genius 3020 – Dual 32L/min Output

Nitrogen generator provides an excellent source of high purity nitrogen gas from air to be used in the LCMS and other instruments at the same time. 

Learn more about he Peak Scientific Nitrogen Generator - Genius 3020 - Dual 32L/min Output.

+ - Microscopes

Nikon Ti-E Inverted Microscope

Nikon A1R Confocal Microscope

Nikon A1R confocal microscope system provides high-resolution imaging of up to 4096 x 4096 pixels with a galvano (non-resonant) scanner and increased light detection efficiency realizes high image quality.

Learn more about the Nikon A1R Confocal Microscope.

Nikon A1R Confocal Microscope

Nikon Ti-E Inverted Microscope

The Nikon Ti-E is a fully motorized for automated multi-mode image techniques and acquisition. The Ti offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. 

Learn more about the Nikon Ti-E Inverted Microscope.

+ - PCR Detection Systems

C11000 touch andnCFX96 detection system

C11000 Touch and CFX96 Touch™ Real-Time PCR Detection System

Bio-Rad CFX96™ Optical Reaction Module converts the C1000 Touch™ Thermal Cycler chassis into the powerful and precise CFX96 Touch Real-Time PCR Detection System.

Learn more about the C11000 Touch and CFX96 Touch Real-Time PCR Detection System.

+ - Spectrofluorophotometers

RF-530 Spectroflourometer

RF-5301PC Spectrofluorophotometer
            
Compared to absorbance methods, fluorescence sensitivity is tens to thousands times better - this means that you can analyze nanogram to picogram samples with great results. Fluorescence can be used also to identify a specific molecule in a complex background. When the compound of interest does not exhibit natural fluorescence, functional group-specific probes may be used to label the compound.

Learn more about RF-5301PC Spectrofluorophotometer.

+ - Spectrometers

Alpha FT-IR Spectrometier

ALPHA FT-IR Spectrometer/Platinum ATR

Bruker Alpha FTIR interferometer with platinum ATR is based on Bruker Optics` patented RockSolid™ design. It offers full FT-IR sampling flexibility. ALPHA's Platinum ATR single reflection diamond ATR module is easy to use for routine analysis. A variety of sampling options for temperature controlled measurements and liquid flow through analysis complete the versatility of the Platinum ATR.

Learn more about ALPHA FT-IR Spectrometer/Platinum ATR.

Bruker Avance III HDTM 400

Bruker Avance III HDTM 400, High Performance Digital Nuclear Magnetic Resonance (NMR) Spectrometer

NMR spectroscopy is used to determine the chemical structures, interaction of molecules, and composition of mixtures. Upgrade includes SampleCASE™ automated sample changer and Prodigy™ ultra-high sensitivity multinuclear Broadband CryoProbe (5 mm sample diameter), with high sensitivity 2H lock channel.

Learn more about the Bruker Avance III HDTM 40, High Performance Digital NMR Spectrometer.

Shimadzu BioSpec Nano machine

Shimadzu BioSpec-nano

Shimadzu BioSpec-nano is a micro-volume UV/VIS spectrophotometer. It offers superior detection limits, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and photometric measurements. Quantitation can be rapidly and simply carried out with very small samples ranging from 1µL-2µL And, with dedicated functions, such as automated sample mounting and wiping.

Learn more about Shimadzu BioSpec-nano.
UV Spectrophotometer machine

UV-2600 UV-Vis Spectrophotometer

Shimadzu UV-2600 is a compact universal, research-grade UV/VIS spectrophotometer that can be used in a wide range of fields, and easily expanded to suit the measurement objective. By using the optional integrating sphere, the measurement wavelength range of the UV-2600 can be extended to the near-infrared region of 1400 nm.

Learn more about the UV-2600 UV-Vis Spectrophotometer.

+ - Mass Spectrometers

Autoflex Spectrometer Autoflex Spectrometer machine

Autoflex Speed MALDI-TOF Mass Spectrometer

Bruker Autoflex Speed MALDI-TOF and TOF/TOF technology is easy-to-use; robust systems for both traditional MALDI applications (reliable and detailed protein/peptide characterization, and polymer analysis) as well as cutting edge work (high resolution MALDI Tissue Imaging, glycan analysis. Further improved smartbeam™-II laser technology with up to 2,000 Hz repetition rate is a must for MALDI Tissue Imaging and delivers superior performance for any Proteomics project.

Learn more about Autoflex Speed MALDI-TOF Mass Spectrometer.
EVOQ Triple Quad Rupole LC-TQ Mass Spectrometer machine

EVOQ Triple Quadrupole LC-TQ Mass Spectrometer

Bruker EVOQ triple quadrupole for liquid chromatography (LC-TQ) is exceptional sensitivity, precision, accuracy, linearity, and a wide dynamic range for your multiple reaction monitoring (MRM) assays. Key application this technology can be used includes, food Testing, environmental Analysis, pharmacokinetics and targeted metabolomics

Learn more about EVOQ Triple Quadrupole LC-TQ Mass Spectrometer.

ImpactII ESI-QTOF Mass Spectrometer machine

Impact II™, ESI–QTOF Mass Spectrometer

Bruker Impact II™ is UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight) mass spectrometry product line with industry-leading > 50,000 Full-Sensitivity Resolution (FSR). It opens up enhanced analytical performance levels for all applications where trace analysis from complex, high-background matrices is a challenge — such as biomarker research, identification of impurities, or residue screening.

Learn more about Impact II, ESI-QTOF Mass Spectrometer.
Shimadzu LCMS 2020 Single Quadrupole Spectrometer

Shimadzu LCMS 2020 Single Quadrupole Liquid Chromatograph Mass Spectrometer (LC/MS)

LCMS is used to separate, detect, characterize, and analyze small molecules, proteins, metabolites, pharmaceuticals, and natural products.

Learn more about the LCMS 2020 Single Quadrupole Liquid Chromatograph Mass Spectrometer.

+ - Synthesizers

Tribute Peptide Synthesizer

Tribute Peptide Synthesizer

Protein Technologies model Tribute peptide synthesizer is a compact benchtop design with chemical-resistant touchscreen and unique Intellisynth UV-Monitoring and Feedback Control system.

Learn more about Tribute Peptide Synthesizer.

+ - X-ray Crystallography

MetalJet x-ray crystallographer

Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction

The METALJET for X-ray diffraction delivers the smallest and most intensive X-ray beam of any home lab X-ray source to meet the ever increasing demands of modern structural biology including protein crystallography and Small Angle X-ray Scattering.  

Learn more about Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction.

+ - Additional Equipment

BioRad Chemidoc Imager machine BioRad ChemiDioc Imager
BioRad Protean 12 IEF Cell Counter machine BioRad Protein i12 IEF Cell for 2D Electrophoresis
BioRad automated Cell Counter machine BioRad TC20 Automated Cell Counter
BioRad transblot turbo gel transfer system machine BioRad TransBlot Turbo Gel Transfer System
Thermost8 Centrifuge machine Thermo ST8 Centrifuge
ThermoSavant Speed Vac Concentrator machine Thermo Savant SpeedVac Concentrator
ThermomaxQ Shaker machine Thermo MaxQ Shaker 

NMR Core Facility

Welcome to the NMR Research Core Facility of School of Pharmacy at the Chapman University. We offer a variety of services to researchers from the Chapman University and for external users. We can provide training and advise researchers how to use NMR spectrometer efficiently. We also can run experiments requested by users.

Fee: Researchers and students of Chapman University have reduced base rates as specified below.  Spectrometer.

For outside users, current base rates are $20/proton and $50/carbon experiment (tube and solvent included.

Our facility is equipped with 400 MHz Bruker NMR spectrometer.

  • 5 mm broadband observe (BBO) probe:
    • 1H (outer coil); 15N-31P and 19F (inner coil).
  • Prodigy broadband observe CryoProbeTM:
    • 1H/19F (outer coil); 15N-31P (inner coil).
  • Z-axis pulsed field gradient capability.
  • Autosampler for automated analysis of up to 24 samples.

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+ - General Rules and Requirements

  • Chapman researchers can use the spectrometer for their own educational or research projects without the charge.
  • Students, assistants, and postdocs, who would like to use the spectrometer, should provide written request from their PI (Chapman University faculty).
  • All users should demonstrate sufficient proficiency in operating the spectrometer. User training is available upon request.
  • All users can analyze their samples with the room-temperature BBO (broadband observe) or Prodigy CryoProbeTM. Please contact Dr. Maslennikov in advance if you need to use the CryoProbe.
  • Please do not bring any radioactive samples – we will not allow to analyze them in any circumstances.
  • All self-service users should provide their own tubes, caps, and NMR-compatible solvents. We recommend Norell 508-UP-7 / Wilmad SP528 or better tubes for quantitative analysis and Norell SVCP-5-178 or better tubes for HTP experiments.
  • Other rules for sample preparation:
    • Use PTFE caps for volatile solvents.
    • Do not use broken tubes and/or damaged caps.
    • Solvent/sample length in the NMR tube: minimum 4 cm, maximum 5 cm.
    • If your tube broke accidently in the magnet, let us know immediately and do not try to recover the tube – the probe can be damaged.
  • If you want to use special tubes, please let us know in advance.
  • We can provide you with appropriate HTP NMR tubes and caps if you return them clean and undamaged.
  • The facility can provide necessary equipment for sample preparation (pipette samplers, tips, hand centrifuge for NMR tubes) and temporary storage (+4C, -20C).
  • If you need any additional equipment to handle your samples, please bring it with you or let us know in advance.

+ - Current Rates for NMR Spectroscopy Services

Internal users self-service: Free of charge.

Internal and External users non-self-service (rates per sample):

 Experiment

Chapman University

Academia and not-for-profit

For-profit
organizations

Remarks

Standard 1D-NMR

1H

$5

$10

$20

 

13

≥4mg

$15

$25

$50

MW <2,000 Da,

1H 1D-spectrum included

<4mg

$35

$70

$150

19F

$20

$35

$70

 

31P

$15

$25

$50

 

Other

$20/hour

$40/hour

$80/hour

Please contact us

1D-NMR binding / interaction analysis

$30/hour

$50/hour

$100/hour

Maximum charge 3h/sample

Homo & Herteo

2D-NMR

$20/hour

$40/hour

$80/hour

Maximum charge 3h/sample

Diffusion 1H

$20

$40

$80

 

Other NMR experiments

Please contact us

 

Spectra Interpretation

$100/hour

Please contact us

+ - Facility Acknowledgement

In the event you use data collected in our Facility for publication, please include  acknowledgment of Chapman University School of Pharmacy NMR Core Facility assistance.

Thank you.